Researchers from the Doctoral Program in Biotechnology, Graduate School, Universitas Padjadjaran, Muhammad Yusuf, and Toto Subroto, have unveiled key insights into optimizing lipid nanoparticle (LNP) formulations for mRNA delivery systems. Employing microfluidic mixing techniques, the team demonstrated that higher lipid concentrations significantly enhance mRNA encapsulation efficiency and ensure LNP particle sizes remain within the critical 80–120 nm range—ideal for efficient immune cell uptake. Additionally, incorporating sucrose as a cryoprotectant proved effective in stabilizing particle size during freeze-thaw storage, especially in formulations with 10 mM lipid and 10 % sucrose even across various temperature conditions.

This work offers substantial contributions to key Sustainable Development Goals (SDGs):

SDG 3 (Good Health and Well-Being): By refining LNP-mRNA stability, the research enhances vaccine reliability and durability, supporting more effective healthcare delivery.

SDG 9 (Industry, Innovation, and Infrastructure): Through innovative use of microfluidic technology and formulation optimization, Unpad is building infrastructure for scalable and accessible drug delivery solutions.

SDG 12 (Responsible Consumption and Production): Fine-tuning lipid and sucrose ratios promotes resource-efficient formulation design, minimizing waste while maximizing functional stability.